Title of article :
Insulin glargine affects the expression of Igf-1r, Insr, and Igf-1 genes in colon and liver of diabetic rats
Author/Authors :
Juárez-Vázquez, Clara I División de Medicina Molecular - Centro de Investigación Biomédica de Occidente - Instituto Mexicano del Seguro Social - Guadalajara - Jalisco, México , Gurrola-Díaz, Carmen M Instituto de Enfermedades Crónico Degenerativas - Departamento de Biología Molecular y Genómica - C.U.C.S - Universidad de Guadalajara - Guadalajara - Jalisco, México , Vargas-Guerrero, Belinda Instituto de Enfermedades Crónico Degenerativas - Departamento de Biología Molecular y Genómica - C.U.C.S - Universidad de Guadalajara - Guadalajara - Jalisco, México , Rosales, José A Domínguez Instituto de Enfermedades Crónico Degenerativas - Departamento de Biología Molecular y Genómica - C.U.C.S - Universidad de Guadalajara - Guadalajara - Jalisco, México , Rodriguez-Ortiz, Jessica F División de Genética - Centro de Investigación Biomédica de Occidente - Instituto Mexicano del Seguro Social - Guadalajara - Jalisco, México , Barros-Núñez, Patricio División de Genética - Centro de Investigación Biomédica de Occidente - Instituto Mexicano del Seguro Social - Guadalajara - Jalisco, México , Flores-Martínez, Silvia E División de Medicina Molecular - Centro de Investigación Biomédica de Occidente - Instituto Mexicano del Seguro Social - Guadalajara - Jalisco, México , Sánchez-Corona, José División de Medicina Molecular - Centro de Investigación Biomédica de Occidente - Instituto Mexicano del Seguro Social - Guadalajara - Jalisco, México , Rosales-Reynoso, Mónica A División de Medicina Molecular - Centro de Investigación Biomédica de Occidente - Instituto Mexicano del Seguro Social - Guadalajara - Jalisco, México
Abstract :
Objective(s): The mitogenic effect of the analogous insulin glargine is currently under debate since several clinical studies have raised the possibility that insulin glargine treatment has a carcinogenic potential in different tissues. This study aimed to evaluate the Igf-1r, Insr, and Igf-1 gene expression in colon and liver of streptozotocin-induced diabetic rats in response to insulin glargine, neutral
protamine Hagedorn (NPH) insulin, and metformin treatments. Materials and Methods: Male Wistar rats were induced during one week with streptozotocin to develop
Type 2 Diabetes (T2D) and then randomly distributed into four groups. T2D rats included in the first group received insulin glargine, the second group received NPH insulin, the third group received metformin; finally, untreated T2D rats were included as the control group. All groups were treated for seven days; after the treatment, tissue samples of liver and colon were obtained. Quantitative PCR (qPCR) was performed to analyze the Igf-1r, Insr and Igf-1 gene expression in each tissue sample.
Results: The liver tissue showed overexpression of the Insr and Igf-1r genes (P>0.001) in rats treated with insulin glargine in comparison with the control group. Similar results were observed for the Insr gene (P>0.011) in colonic tissue of rats treated with insulin glargine. Conclusion: These observations demonstrate that insulin glargine promote an excess of insulin and IGF-1 receptors in STZ-induced diabetic rats, which could overstimulate the mitogenic signaling pathways.
Keywords :
Colon , Diabetes , Insulin glargine , Liver , Metformin , NPH insulin , Rats
