Title of article :
Hydatidosis: The Time Required for Protoscolices Implantation in the Peritoneal Cavity of Rats
Author/Authors :
Mohtarami, Abolfazl Department of Surgery - Valiasr Hospital - Arak University of Medical Sciences, Arak , Amini, Mahmoud Department of Surgery - Valiasr Hospital - Arak University of Medical Sciences, Arak , Hasanpour, Akbar Department of Pathology - Valiasr Hospital - Arak University of Medical Sciences, Arak , Eslami, Zahra Department of Parasitology - School of Medicine - Arak University of Medical Sciences, Arak
Abstract :
Background: Hydatid disease is a parasitic
infection caused by the larval stage of E.
granulosus whose life cycle involves livestock
and dogs as intermediate and definitive hosts,
respectively. Surgery is a common treatment
for hydatid cysts but rupture of cyst and
spillage of the contents may cause secondary
implantation of cysts. The objective of this
study was to determine the time required for
protoscolices implantation in different organs.
Materials and Methods: Thirty-six rats were
divided into six groups of 6 animals each. The
rats in five groups were injected
intraperitoneally with 2 milliliters of hydatid
fluid containing 100 protoscolices but the rats
in sixth group (as control group) were injected
with 2 milliliters of normal saline. The rats in
groups I, II, III, IV and V were sacrificed and
opened after 1, 24, 48 , 72 and 168 hours,
respectively. All internal organs were evaluated
carefully and 3 samples from liver, omentum
and intestine were prepared for light
microscopy.
Results: There was no implantation of
protoscolices in the tissues of first, second and
third groups of rats. But protoscolices were
implanted in tissue samples of fourth and fifth
group that were sacrificed 72 and 168 hours
after injection.
Conclusion: The protoscolices of hydatid cyst
need at least 72 hours for implantation after
intraperitoneal spillage.
Keywords :
Echinococcosis , Intraperitoneal Injections , Rats
Journal title :
Astroparticle Physics