Title of article :
Antimicrobial Photothermal Treatment of Pseudomonas Aeruginosa by a Carbon Nanoparticles-Polypyrrole Nanocomposite
Author/Authors :
Behzadpour, N Department of Medical Physics - School of Medicine - Shiraz University of Medical Sciences, Shiraz, Iran , Sattarahmady, N Department of Medical Physics - School of Medicine - Shiraz University of Medical Sciences, Shiraz, Iran , Akbari, N Department of Microbiology - Faculty of Science - Arak Branch Islamic Azad University, Arak, Iran
Abstract :
Background: Nowadays, it is needed to explore new routes to treat infectious
bacterial pathogens due to prevalence of antibiotic-resistant bacteria. Antimicrobial
photothermal therapy (PTT), as a new strategy, eradicates pathogenic bacteria.
Objective: In this study, the antimicrobial effects of a carbon nanoparticles-polypyrrole
nanocomposite (C-PPy) upon laser irradiation were investigated to destroy
the pathogenic gram-negative Pseudomonas aeruginosa.
Material and Methods: In this experimental study, the bacterial cells were
incubated with 50, 100 and 250 μg mL-1 concentrations of C-PPy and irradiated with
a 808-nm laser at two power densities of 0.5 and 1.0 W cm-2. CFU numbers were
counted for the irradiated cells, and compared to an untreated sample (kept in dark).
To explore the antibacterial properties and mechanism(s) of C-PPy, temperature
increment, reactive oxygen species formation, and protein and DNA leakages were
evaluated. Field emission scanning electron microscopy was also employed to investigate
morphological changes in the bacterial cell structures.
Results: The results showed that following C-PPy attachment to the bacteria
surface, irradiation of near-infrared light resulted in a significant decrement in the
bacterial cell viability due to photothermal lysis. Slightly increase in protein leakage
and significantly increase intracellular reactive oxygen species (ROS) were observed
in the bacteria upon treating with C-PPy.
Conclusion: Photo-ablation strategy is a new minimally invasive and inexpensive
method without overdose risk manner for combat with bacteria.
Keywords :
Nanocomposites , Polypyrrole , Pseudomonas aeruginosa
Journal title :
Journal of Biomedical Physics and Engineering