Frequency of Extend Spectrum β-Lactamases Genes among Klebsiella Pneumoniae Strains Isolated from Patients Admitted to Shahid Beheshti Hospital, Babol City, Iran

Background: Regarding the emergence and increasing of multidrugresistant strains among Klebsiella pneumoniae nosocomial isolates the therapeutic options for the treatment has been limited. The β-lactamases enzymes are the major defense of gramnegative bacteria against antibiotics. The aim of this study was to the detection of bla_CTXM, bla_SHV, and bla_TEM genes among K. pneumoniae strains isolated from patients who admitted to Shahid Beheshti hospital of Babol city, Iran, in 2016.Materials and Methods: This study was conducted on 50 samples of K. pneumoniae strains isolated from hospitalized patients. Antibiotic susceptibility testing was performed by KirbyBauer disc diffusion method according to CLSI guidelines. The bla_CTXM, bla_SHV, and bla_TEM genes were detected by polymerase chain reaction method.Results: Among studied strains, the prevalence of bla_CTXM, bla_SHV, and bla_TEM genes were 24 (49%), 44 (88%), and 36 (72%), respectively. In this study, imipenem and nitrofurantoin were more effective than other antibiotics. Also, 100% of strains were susceptible to imipenem.Conclusions: The prevalence of antibiotic resistance genes detected in this study implies a great concern for the treatment of multidrugresistant K. pneumonie. Hence, infection control measures, including antibacterial management and identification of resistant isolates for preventing of nosocomial outbreaks have become highlighted.