Author/Authors :
Mohammadi, F. tehran university of medical sciences tums - Molecular Immunology Research Center, Faculty of Medicine - Department of Immunology,, تهران, ايران , Niknam, M. H. tehran university of medical sciences tums - Molecular Immunology Research Center, Faculty of Medicine - Department of Immunology, تهران, ايران , Nafar, M. shahid beheshti university of medical sciences - Shahid Labbafinejad Hospital - Department of Nephrology, تهران, ايران , Einollahi, B. baqiyatallah university of medical sciences - Nephrology Research Center, Faculty of Medicine, ايران , Nazari, B. tehran university of medical sciences tums - Molecular Immunology Research Center, Faculty of Medicine - Department of Immunology, تهران, ايران , Lessanpezeshki, M. tehran university of medical sciences tums - Nephrology Research Center, Faculty of Medicine, تهران, ايران , Amirzargar, M. A. hamadan university of medical sciences - Faculty of Medicine - Department of Immunology, ايران , Solgi, G. hamadan university of medical sciences - Faculty of Medicine - Department of Immunology, ايران , Nikbin, B. tehran university of medical sciences tums - Molecular Immunology Research Center, Faculty of Medicin - Department of Immunologye, تهران, ايران , Amirzargar, A. A. tehran university of medical sciences tums - Molecular Immunology Research Center, Faculty of Medicin - Immunogenetics Lab, Department of Immunologye, تهران, ايران
Abstract :
Background: A growing body of evidence demonstrated an immune etiology as well as nonimmune mechanisms for episodes of clinical acute rejection and long-term allograft dysfunction. Objective: To investigate the correlation of IFN-γ-producing cells and TGF-β with incidence of clinical acute rejection in living-related and unrelated kidney allogarft recipients during the first post-transplant year. Methods: This multi-center study was performed on 57 kidney allograft recipients from living- related (n=20) and unrelated (n=37) donors between April 2011 and September 2012 and who were followed prospectively for a mean period of one year. Peripheral blood samples were collected from all patients pre-transplantation and at days 14, 30 and 90 after transplantation; PBMCs were used as responding cells in enzyme-linked immunosorbent spot (ELISPOT) assay to measure the frequency of IFN-γ-producing cells after stimulation with donor lymphocytes. Additionally, TGF-β levels were measured in cell culture supernatants of ELISPOT assay. Results: During the follow-up period, 45 (79%) patients were diagnosed with stable graft function (group A); 12 (21%) experienced clinical acute rejection episodes (group B). The frequency of IFN-γ-producing cells was significantly (p 0.001) higher in the rejection group in all three times after transplantation. Also, post- transplantation comparison for TGF-β showed a significantly (p 0.001) higher contents in group A vs. group B. Comparing the post-transplantation levels of TGF-β and mean numbers of IFN-γ- producing cells between groups A and B demonstrated a continuous increment in TGF-β and decreasing frequencies of IFN-γ-producing cells in group A vs. group B. Conclusion: Serial post-transplantation monitoring of IFN-γ-producing donor reactive cells during the first months is a clinically feasible approach for identification of kidney allogarft recipients at risk for ongoing immune-mediated graft damage and later graft loss.
Keywords :
Kidney , Allograft , TGF , β , IFN , γ
