Title of article :
Solid Phase Extraction of Letrozole by a Metal Organic Framework and its HPLC-UV Characterization
Author/Authors :
Mohammadi, S. Nano Drug Delivery Research Center - Health Technollogy Institute - Kermanshah University of Medical Sciences, Kermanshah, Iran , Hazrati, M. Nano Drug Delivery Research Center - Health Technollogy Institute - Kermanshah University of Medical Sciences, Kermanshah, Iran , Yamini, Y. Department of Chemistry - Tarbiat Modares University - P. O. Box: 14115-175 - Tehran, Iran
Abstract :
We successfully synthesized TMU-24 as metal-organic framework (MOF) and employed as an effective sorbent for preconcentration of
letrozole (LTZ) from biological samples. Using high-performance liquid chromatography with UV detector (HPLC-UV) coupling with
solid-phase extraction (SPE), a sensitive, fast, and simple method for detection of LTZ has been developed. The optimal conditions, such as
pH, type of eluent solvents, contact time, amount of adsorbent, and adsorption capacity are discussed. Under the optimized conditions,
relative recoveries (RR) and preconcentration factor (PF) of the LTZ was obtained in the range of 92.4-99.6% and 33, respectively. Under
the optimal conditions, LTZ can be determined with a limit of detection (LODs) 0.5 μg l-1 (based on S/N = 3), a linearity in the range of
1-250 μg l-1, and a reasonable linearity of 0.995. Also, the experimental adsorption isotherms models were investigated. The developed
method was successfully used to the analysis of LTZ in urine samples.
Keywords :
TMU-24 , Metal-organic framework , Solid-phase extraction , Letrozole , HPLC-UV
Journal title :
Analytical and Bioanalytical Chemistry Research
