Title of article :
Phytochemical and Biological Investigation of Nitraria retusa Asch
Author/Authors :
El-Alali, Abdullah Muta’h University, Jordan , Al Zoubi, Abdullah Muta’h University, Jordan , Gharaibeh, Mohammad Jordan University of Science and Technology - Fuclty of Agriculture, JUST, Jordan , Tawaha, Khaled University of Jordan, Jordan , Alali, Feras Q. Jordan University of Science and Technology - Faculty of Pharmacy, Jordan
Abstract :
Nitraria retusa Asch (Zygophyllaceae) of Jordanian origin has been investigated phytochemically andbiologically. Three compounds, two new and one previously known, have been isolated and identified. The newcompounds are 5,7-dihydroxy-3-deoxy vasicine (I) and 7-hydroxy-3-deoxy-1-vasiciene (II), while the knowncompound is O-acetyl nitraraine (I) but reported for the first time from this species. The relative levels ofantioxidant activity and total phenolic content of the methanolic extract of Nitraria retusa and fraction B (thealkaloid rich fraction) were determined using the improved ABTS•+ method and the Folin-Ciocalteu colorimetricmethod, respectively. The total phenolic content of the methanolic extract and fraction B was found to be 234.66and 114.53 mg GAE/g extract, respectively, while the total antioxidant capacity was found to be 429.3 and 809.9μmol TE/g extract, respectively. The methanolic extract and fraction B were also tested for their generalcytotoxicity using the brine shrimp lethality test (BSLT),but no activity was detected (LC50 1000 ppm)
Keywords :
Nitraria retusa , Al , Ghardaq , antioxidant activity , total phenolic content , Zygophyllaceae
Journal title :
Jordan Journal of Pharmaceutical Sciences
Journal title :
Jordan Journal of Pharmaceutical Sciences
