Title of article :
Molecular and Serological Characterization of Toxoplasma gondii in Women in Wasit Province
Author/Authors :
Al-Sray, Abbas Hassan Department of Microbiology - Veterinary Medicine College - Wasit University, Wasit, Iraq , Rashid Sarhan, Sarhan Department of Physiology and Pharmacology - College of Veterinary Medicine - Wasit University, Wasit, Iraq , Mohammed, Hussein Ali Department of Microbiology - Veterinary Medicine College - Wasit University, Wasit, Iraq
Abstract :
The present study was conducted during the period from October 2013 to May 2014 in Wasit Province to
detect the T. gondii in women using molecular and serological methods. Five hundred blood samples and 8 placenta
specimens were collected from suspected women. The sera samples were separated and examined by ELISA to detect
Toxoplasmosis serologically. In addition 89 blood samples and 8 placental tissue samples were subjected to polymerase
chain reaction (PCR) technique to detect for molecular identification. The Serological results showed that 17.8% of
women were positive for the private screening ELISA detects toxoplasmosis (17 % with chronic infection and 0.8%
with acute infection). Moreover, it was noted that the highest rate of infection was in women who ranged in age
between 20-29 years, reaching 19 .9%, but with no significant difference (P>0.05) between the ages studied. The study
indicated a lack of months effect on the distribution of parasite infection rates where these different months recorded
relatively close rate ranged between 14.45% - 23.07% with no significant difference (P<0.05). Regarding to polymerase
chain reaction test, when a fragment of 399bp was amplified from B1 gene, the result showed that 6.74% of blood
samples and 100% of placental tissue samples were positive to this test. In Conclusion the Toxoplasma infection in
women was relatively high in Wasit Province.
Keywords :
Toxoplasma gondii , ELISA , B1gene , PCR
Journal title :
Advances in Animal and Veterinary Sciences