Author/Authors :
Haron, Norhisham Universiti Teknologi MARA - Faculty of Health Sciences - Department of Medical Laboratory Technology, Malaysia , Abu Hasan, Nuraishah Universiti Teknologi MARA - Faculty of Health Sciences - Department of Medical Laboratory Technology, Malaysia , Camalxaman, Siti Nazrina Universiti Teknologi Mara, Puncak Alam Campus - Faculty Of Health Sciences - Department of Medical Laboratory Technology, Malaysia , Mohamed, Emida Universiti Teknologi MARA - Faculty of Health Sciences - Department of Medical Laboratory Technology, Malaysia , Rambely, Azlin Sham Universiti Teknologi MARA - Faculty of Health Sciences - Department of Medical Laboratory Technology, Malaysia , Amom, Zulkhairi Universiti Teknologi MARA - Faculty of Health Sciences - Department of Basic Science, Malaysia
Abstract :
Stevia rebaudiana, a member of Asteraceae family is rich in stevioside and total phenolic compounds. The aim of the present study was to investigate the protective effect of Stevia rebaudiana leaf extract on human erythrocytes against oxidative damage, in vitro. The protective effect of Stevia rebaudiana leaf extract in erythrocytes was studied in terms of resistance to hemolysis, lipid peroxidation and morphological changes. Stevia rebaudiana leaf extract at different concentrations (100, 200 and 300 μg/mL) was tested in 10 mM hydrogen peroxide (H2O2) induced oxidative stress on human erythrocytes. The hemolysis of erythrocytes induced by H2O2 was inhibited in a dose-dependent manner. The Stevia rebaudiana leaf extract was found to inhibit erythrocyte’s lipid peroxidation caused by H2O2. Morphological alterations in erythrocytes induced by H2O2 were protected by the leaf extract. In conclusion, the study demonstrated that Stevia rebaudiana leaf extract protected erythrocytes against oxidative damage due to its antioxidant properties.
Keywords :
Stevia rebaudiana extract , antioxidant , human erythrocytes , oxidative stress , hemolysis
