Author/Authors :
Akhtar, Riaz Department of Mechanical - Materials and Aerospace Engineering - School of Engineering - University of Liverpool - L69 3GH, UK , Chang, Zhuo Department of Mechanical - Materials and Aerospace Engineering - School of Engineering - University of Liverpool - L69 3GH, UK , Paoletti, Paolo Department of Mechanical - Materials and Aerospace Engineering - School of Engineering - University of Liverpool - L69 3GH, UK , Hansen, Maria Lyck Department of Clinical Biochemistry and Pharmacology - Center for Individualized Medicine in Arterial Diseases - Odense University Hospital - University of Southern Denmark, Denmark , Beck, Hans Christian Department of Clinical Biochemistry and Pharmacology - Center for Individualized Medicine in Arterial Diseases - Odense University Hospital - University of Southern Denmark, Denmark , Rasmussen, Lars Melholt Department of Clinical Biochemistry and Pharmacology - Center for Individualized Medicine in Arterial Diseases - Odense University Hospital - University of Southern Denmark, Denmark , Chen, Po-Yu Department of Materials Science and Engineering - National Tsing Hua University - Hsinchu 30013, Taiwan
Abstract :
Usingtheatomicforcemicroscopy-(AFM-)PeakForcequantitativenanomechanicalmapping(QNM)technique,wehavepreviouslyshownthattheadventitiaofthehumaninternalmammaryartery(IMA),testedunderdehydratedconditions,isalteredinpatientswithahighdegreeofarterialstiffening.Inthisstudy,weexploredthenanoscaleelasticmodulusofthetunicamediaoftheIMAinhydratedanddehydratedconditionsfromthepatientswithlowandhigharterialstiffening,asassessedin vivobycarotid-femoralpulsewavevelocity(PWV).Inbothhydratedanddehydratedconditions,themediallayerwassignificantlystifferinthehighPWVgroup.TheelasticmodulusofthehydratedanddehydratedtunicamediawassignificantlycorrelatedwithPWV.Inthehydratedcondition,theexpressionactivityofcertainsmallleucine-richrepeatproteoglycans(SLRPs),whichareassociatedwitharterialstiffening,werefoundtobenegativelycorrelatedtothemedialelasticmodulus.WealsocomparedthedatawithourpreviousworkontheIMAadventitia.WefoundthatthehydratedmediaanddehydratedadventitiaarebothsuitableforreflectingthedevelopmentofarterialstiffeningandSLRPexpression.ThiscomprehensivestudyofthenanomechanicalpropertiesintegratedwiththeproteomicanalysisintheIMAsdemonstratesthepossibilityoflinkingstructuralpropertiesandfunctioninsmallbiologicalsampleswithnovelAFMmethods.TheIMAisasuitabletargetforpredictingarterialstiffening.
Keywords :
AFM Characterization , Internal Mammary Artery , Novel Target , Arterial Stiffening , PWV , tomicforcemicroscopy-(AFM-) , QNM
