Author/Authors :
Telegin, G.B Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino, Russia , Minakov, A.N Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino, Russia , Chernov, A.S Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino, Russia , Manskikh, V.N M.V. Lomonosov Moscow State University, Moscow, Russia , Asyutin, D.S N.N. Burdenko National Scientific and Practical Center for Neurosurgery - RF Health Ministry, Moscow, Russia , Konovalov, N.A N.N. Burdenko National Scientific and Practical Center for Neurosurgery - RF Health Ministry, Moscow, Russia , Gabibov, A.G Shemyakin-Ovchinnikov Institute of bioorganic chemistry, Moscow, Russia
Abstract :
We developed and verified an original, minimally invasive method for surgical simulation of a posttraumatic spinal cord glial scar in rats. The model is intended for use as a biological platform for testing the stimulation of regenerative processes in the central nervous system. Unification of the model enables one to achieve versatility both for implantation techniques and for the development of system-action approaches. Faced with a standard structural defect of the spinal cord, researchers will have the unique opportunity to test
in vivo promising methods for spinal function recovery in the posttraumatic period. We developed anesthetic support, surgical tactics, and a set of rehabilitation measures for the chronic postoperative period. Experimental exposure effects were preliminarily assessed in vivo using a standard technique for recording the motor activity of rats in the postoperative period of spinal cord injury. Our final conclusions were drawn based on an analysis of histological sections of the rat spinal cord glial scar in three mutually perpendicular planes.
Keywords :
unilateral hemilaminectomy , cryoapplica-tion , axonal regeneration , glial scar , spinal cord injury , laboratory rat , surgical simulation
