Title of article :
The role of histone deacetylase inhibitors in regulation of Akt/GSK-3β signaling pathway in mice following transient focal cerebral ischemia
Author/Authors :
Zhao, Bo Department of Anesthesiology - Wuhan University, China , Liu, Lian Department of Anesthesiology - Wuhan University, China , Leng, Yan Department of Anesthesiology - Wuhan University, China , Yuan, Quan Department of Anesthesiology - Wuhan University, China , Hou, Jiabao Department of Anesthesiology - Wuhan University, China , Wu, Yang Department of Anesthesiology - Wuhan University, China , Gao, Wenwei Department of Critical Care Medicine - Wuhan University, China
Pages :
11
From page :
862
To page :
872
Abstract :
Purpose: To investigate whether the neuroprotective effect of TSA on cerebral ischemia reperfusion injury is mediated by the activation of Akt/GSK-3β signaling pathway. Methods: Mice were randomly divided into four groups (n=15): sham group (S); ischemia reperfusion group (IR); ischemia reperfusion and pretreated with TSA group (IR+T); ischemia reperfusion and pretreated with TSA and LY294002 group (IR+T+L). The model of cerebral ischemia reperfusion was established by 1h of MCAO following 24h of reperfusion. TSA (5mg/kg) was intraperitoneally given for 3 days before MCAO, Akt inhibitor, LY294002 (15 nmol/kg) was injected by tail vein 30 min before the MCAO. Results: TSA significantly increased the expression of p-Akt, p-GSK-3β proteins and the levels of SOD, Bcl-2, reduced the infarct volume and the levels of MDA, ROS, TNF-α, IL-1β, Bax, Caspase-3, TUNEL and attenuated neurological deficit in mice with transient MCAO, LY294002 weakened such effect of TSA dramatically. Conclusions: TSA could significantly decrease the neurological deficit and reduce the cerebral infarct volume, oxidative stress, inflammation, as well as apoptosis during cerebral ischemia reperfusion injury, which was achieved by activation of the Akt/GSK-3β signaling pathway.
Keywords :
Histone Deacetylases , Glycogen Synthase Kinase 3 beta , Brain Ischemia , Reperfusion Injury , Mice
Journal title :
Acta Cirurgica Brasileira
Serial Year :
2017
Full Text URL :
Record number :
2617979
Link To Document :
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