Author/Authors :
Luo, Shengjuan Department of Ultrasound - The Tird Xiangya Hospital - Central South University - Changsha, China , Ding, Jinsong School of Pharmaceutical Sciences - Central South University - Changsha, China , Wang, Peiqi Department of Pharmacy - Cancer Hospital of Henan Province - Zhengzhou, China , Wang, Zheng Department of Hepatobiliary Surgery - The Third Xiangya Hospital - Central South University - Changsha, China , Ma, Xiaoqian Department of Radiology - The Third Xiangya Hospital - Central South University - Changsha, China , Yang, Cejun Department of Radiology - The Third Xiangya Hospital - Central South University - Changsha, China , Liang, Qi Department of Radiology - The Third Xiangya Hospital - Central South University - Changsha, China , Rong, Pengfei Department of Radiology - The Third Xiangya Hospital - Central South University - Changsha, China , Wang, Wei Department of Radiology - The Third Xiangya Hospital - Central South University - Changsha, China
Abstract :
Perfuorooctyl bromide (PFOB) enclosed nanoparticles (NPs) as ultrasonic contrasts have shown promising results in the recent
years. However, NPs display poor contrast enhancement in vivo. In this work, we used the copolymers poly(lactide-co-glycolide)
carboxylic acid (PLGA-COOH) and poly(lactide-co- glycolide) poly(ethylene glycol) carboxylic acid (PLGA-PEG-COOH) as a
shell to encapsulate PFOB to prepare a nanoultrasonic contrast agent. The NPs were small and uniform (210.6 ± 2.9 nm with
a polydispersity index of 0.129 ± 0.016) with a complete shell nuclear structure under the transmission electron microscopy
(TEM). In vitro, when concentration of NPs was ≥10 mg/ml and clinical diagnostic frequency was ≥9 MHz, NPs produced intensive
enhancement of ultrasonic gray-scale signals. NPs could produce stable and obvious gray enhancement with high mechanical index
(MI) (MI > 0.6). In vivo, the NPs ofered good ultrasound enhancement in tumor after more than 24 h and optical imaging also
indicated that NPs were mainly located at tumor site. Subsequent analysis confrmed that large accumulation of fluorescence was
observed in the frozen section of the tumor tissue. All these results caused the conclusion that NPs encapsulated PFOB has achieved
tumor-selective imaging in vivo.
Keywords :
Carboxyl , Perfluorooctyl , TEM , UCA
