Author/Authors :
Khayoon, Wejdan Shakir Universiti Sains Malaysia - School of Chemical Sciences, Malaysia , Al-Abaichy, Mouyad Qassim Baghdad University - College of Science - Department of Chemistry, Iraq , Jasim, Mohamed Baghdad University - College of Science - Department of Chemistry, Iraq , Al-Hamadany, Mohamad Affan Baghdad University - College of Science - Department of Chemistry, Iraq
Abstract :
A new spectrophotometric method for the determination of allopurinol drug was investigated. The proposed method was based on the reaction of the intended drug with catechol and Fe(II) to form a blue soluble complex which was measured at ëmax 580 nm. A graph of absorbance versus concentration shown that Beer’s law was obeyed over the concentration range of 2–10 µg ml–1 with molar absorptivity of 9.4 x 103 l mol–1 cm–1 and Sandell sensitivity of 1.4 x 10–2 µg cm–2. A recovery percentage of 100% with RSD of 1.0%–1.3% was obtained. The proposed method was applied successfully for the determination of allopurinol drug in tablets with a good accuracy and precision. The optimum condition for the color development has also been investigated.
