Micropropagation of Asam Karanda (Carissa carandas Linn)

An efficient micropropagation protocol was developed for C. carandas using nodal segment as the explants. Murashige and Skoog medium (MS), supplemented with 2.0 – 4.0 mg/L Benzylaminopurine (BA), induced an average of 3.6 - 5.5 shoots/explants but the number of multiple shoots formed was not significantly different. However, the addition of IBA combined with BA had shown a reducing trend in the number of shoot being formed. The MS medium supplemented with 2.0 mg/L 3-Indolebutyric acid (IBA) was found to be effective for rooting of micro-shoots. After the acclimatization process, 84% ± 4.3% of the in vitro plantlets survived but there was a reduction in the number of survived plantlets (59 ± 3.7%) after 4 weeks of incubation in the green house condition.