Title of article :
Heat shock proteins enriched-promastigotes of leishmania major inducing Th2 immune response in BALB/c Mice
Author/Authors :
Holakuyee, Marzieh pasteur institute of iran - Department of immunology, ايران , Mandavi, Mehdi pasteur institute of iran - department of virology, ايران , Mohammad Hassan, Zuhair tarbiat modares university - faculty of medical sciences - department of immunology, تهران, ايران , Abolhassani, Mohsen pasteur institute of iran - department of immunology, ايران
From page :
209
To page :
217
Abstract :
Background: Heat shock proteins (HSP) are highly conserved molecules with many immunological functions. They are highly immunogenic with important role in cancer immunotherapy and in vaccine development against infectious diseases. As adjuvant, HSP can augment the immunogenicity of weak antigens and can stimulate antigen presenting cells. Although vaccines have been successful for many infectious diseases, progress in leishmaniasis has not been achieved. In this report, the protective effect of HSP-enriched soluble leishmania antigen (SLA) was determined. Methods: BALB/c mice were immunized 3x with HSP-enriched SLA and SLA alone and 10 days after final boost. They were infected with 106 stationary phase promastigote of Leishmania major and immunological responses were followed until nine weeks. Results: No significant differences were observed in lymphocyte proliferation, footpad swelling, parasite burden, nitric oxide or IL-12 cytokine between HSP-enriched or SLA groups. Although the levels of IFN-γ, IL-4, TGF-β, IgGI and IgG2b were increased in both groups, IFN-γ was significantly higher in SLA group and IgG2a in HSP-enriched SLA. Conclusion: These results indicate that HSP direct the immune system towards Th2 pattern and does not have protective role in L. major infection.
Keywords :
Adjuvant , Heat shock proteins (HSP) , Leishmaniasis
Journal title :
Iranian Biomedical Journal(IBJ)
Journal title :
Iranian Biomedical Journal(IBJ)
Record number :
2668345
Link To Document :
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