Title of article :
تقنية انزيم اليوريز المستخلص من بذور الرقي
Author/Authors :
الراوي, انيس مالك جامعة بغداد - كلية العلوم للبنات, العراق , طه, محمد مبارك علي وزارة التعليم العالي و البحث العلمي, العراق , خليل, فيحاء مقداد جامعة بغداد - كلية العلوم للبنات, العراق
Abstract :
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Other languages abstract :
Watermelon seeds showed also a higher production even more than the legumes.
Measured enzyme activity for watermelon milled seeds was found to have 353 unit/gm seeds.
However, stabilization characters were studied which showed that there is a very high stability under extraction conditions and under different storage parameters.
Results also showed that the enzyme stability decreased gradually in the presence of 2-mercaptoethanol, ethylene diamine letra acetate di sodium (EDTA) at (1 mmoiar) for each concentration.
Addition of glycerol (10%) to a buffer's solution used is the process of enzyme activity.
Enzyme from C.
vulgaris was purified through different steps as extraction, dialysis, heat treatment, acetone fractionation, ionic exchange as a (Batch wise) employing ion exchange column using diethyl amino ethyl cellulose (DEAE cellulose),.
Separation results showed that the enzyme found lo have two shapes (A & B) and it can be easil separatcdfrom each other, using NaCI (0.2 and 0.3) Molar subsequently.
The specific activity were measured and found to be (14L66) and (58.57 unit/mg protein) since the activity of enzyme A is superies that of enzymes by two folds.
Purification steps for enzyme A were completed through filtration on the column of Sepbadex G-200 and the specific activity for this purified enzyme reached up to 204.54 unit/mg protein.
Keywords :
انزيم اليوريز , بذور الرقي , المستخلص
Journal title :
Baghdad Science Journal
Journal title :
Baghdad Science Journal
