synthesis and study impaction antibacterial, antifungal activity newly pyridazine and 1,2,4-triazine derivatives

in this research study, the synthesis sequence of novel pyridazine and 1,2,4-triazine derivatives was determined by reacting α-hydrazino-ncarbazole acetamide (1) with acid anhydride derivatives used glacial acetic acid as a solvent to prepare compounds (2-9) pyridazine derivatives reacted compound (1) with phenyl isocyanate, phenyl thioisocyanate, and α-naphthyl isocyanate to use absolute ethanol as solvent to give compounds (10-12). compounds (10-12) were condensed with (2n. naoh) to give compounds (14-16) to denominate 1,2,4-triazine derivatives. reacted compound (1) with cs2/koh used absolute ethanol as solvent to provide potassium salt (13). this salt reacts with 95% hydrazine hydrate to give compound (17). the newly synthesized compounds were tested against different microorganisms to evaluate their antimicrobial activities on bacterial strains, gram-positive bacteria, gram-negative bacteria, and fungal strains to identify the most efficient biologically active compounds.