Title of article :
The effect of different doses of GnRH on stress responses in female koi carp (Cyprinus carpio)
Author/Authors :
Eslami, M. M Department of Fisheries - Qaemshahr Branch - Islamic Azad University - Qaemshahr, Iran , Javadian, S. R Department of Fisheries - Qaemshahr Branch - Islamic Azad University - Qaemshahr, Iran , Bahram, S Department of Fisheries - Qaemshahr Branch - Islamic Azad University - Qaemshahr, Iran
Abstract :
This study was conducted to evaluate the effect
of different doses of GnRH on stress response
in female koi carp (Cyprinus carpio). For this
purpose, forty sexually mature female koi carp
with an average weight of 102.05 ± 9.03 g were
divided to four groups and fish received with a
single intraperitoneal 0.9% NaCl with 20 mg
kg-1 metoclopramide (C); 10 μg kg body
weight-1 (BW) GnRH with 20 mg kg-1
metoclopramide (Gn10); 20 μg kg BW-1 GnRH
with 20 mg kg-1 metoclopramide (Gn20); 50 μg
kg BW-1 GnRH with 20 mg kg-1 metoclopramide
(Gn50). Blood samples were collected before
and after ovulation (approximately 24 h postinjection).
The concentrations of plasma
cortisol, lactate, and glucose were measured.
Broodstocks who received GnRH spawned,
while broodstocks of the group C did not
spawn. The Gn10, Gn20, and Gn50 treatments
led to significantly higher cortisol, lactate, and
glucose concentration after ovulation compared
to before injection (p< 0.05).The present results showed that females are
highly sensitive to manipulation during
reproduction and higher levels of the hormone
cause more stress broodstocks, so they must be
held with minimal disorders, especially during
spawning period.
Keywords :
Cyprinus carpio , GnRH , Artificial spawning , Hormonal treatment , Cortisol
Journal title :
Iranian Journal of Aquatic Animal Health