Title of article :
Clinical Application Study of Polymeric Nanospheres Network in Methylphenidate Extraction from Urine Samples by Dispersive Solid Phase Extraction Adsorbent
Author/Authors :
Taghvimi, Arezou Biotechnology Research Centre - Tabriz University of Medical Science, Tabriz, Iran , Soghra Jahed, Fatemeh Department of chemistry - Faculty of Science - Azarbijan Shahid Madani University, Tabriz, Iran , Dastmalchi, Siavoush Biotechnology Research Centre - Tabriz University of Medical Science, Tabriz, Iran , Javadzadeh, Yousef Faculty of Pharmacy - Tabriz University of Medical Science, Tabriz, Iran
Abstract :
Purpose: This research introduces a polymeric nanosphere as a new dispersive solid phase
extraction (DSPE) adsorbent for the extraction of methylphenidate (MPH) from urine and its
high performance liquid chromatography (HPLC) analysis.
Methods: Polymeric nanosphere is a kind of copolymeric network obtained by copolymerization
of an ionic liquid monomer and styrene in the presence of vinyltriethoxysilane and
2-hydroxyethylmethacrylate. HPLC coupled with ultra violet detector was applied for the
determination and quantification of MPH. Dominant parameters in extraction were modified by
the one-parameter-at-a-time method. The results are as follow: 10 mg of polymeric nanospheres
(PNS), 400 μL of acetonitrile (ACT), 5 mL of urine with the pH value of 9, and the extraction
and desorption times of 2 and 5 minutes, respectively, which can be selected as the optimum
extraction conditions.
Results: Calibration curve was plotted through optimized conditions, and the proposed method
was validated. The results demonstrated that the method presented linearity in the concentration
range of 30-1200 ng/mL. Selectivity, matrix effect and metabolites interference effect were
investigated and the method presented no obvious interference effect during the analysis run
time. Repeatability, limit of detection (LOD) and limit of quantification (LOQ) values of the
method can be reported in this section as well. The method showed satisfactory results with
98.8% relative recovery in the analysis of positive urine samples.
Conclusion: The findings convinced the applicability of the introduced method for DSPE and
HPLC analysis of the positive urine samples in different laboratories.
Keywords :
Polymeric nanosphere , Methylphenidate , Dispersive solid phase extraction , Urine
Journal title :
Advanced Pharmaceutical Bulletin