Author/Authors :
Kapitonova, Marina Faculty of Medicine and Health Sciences - UNIMAS - Kota Samarahan - Sarawak, Malaysia , Gupalo, Sergey Faculty of Medicine MAHSA University - Bandar Saujana Putra - Jenjarom - Selangor, Malaysia , Alyautdin, Renad Medicinal Products Safety - Scientific Centre for Expert Evaluation of Medicinal Products - Moscow, Russia - Department of Pharmacology - Sechenov University - Moscow, Russia , Aziz Ibrahim, Ibrahim Abdel Department of Pharmacology and Toxicology - Faculty of Medicine - Umm Al-Qura University - Makkah, Saudi Arabia , Salim, Norita Institute of Medical Molecular Biotechnology and Faculty of Medicine - UiTM - Sungai Buloh - Selangor, Malaysia , Ahmad, Azhar Institute of Medical Molecular Biotechnology and Faculty of Medicine - UiTM - Sungai Buloh - Selangor, Malaysia , Bahri Talip, Saiful Faculty of Medicine and Health Sciences - UNIMAS - Kota Samarahan - Sarawak, Malaysia , Moe Nwe, Tin Faculty of Medicine and Health Sciences - UNIMAS - Kota Samarahan - Sarawak, Malaysia , Morokhina, Svetlana Department of Pharmacognosy - Sechenov University - Moscow, Russia
Abstract :
Objective: Modern treatment of peptic ulcers includes antibacterial and gastroprotective medications. However, current anti-ulcer drugs possess severe side effects. Therefore, all attempts to find new effective medications free from side effects are justified. Though Berberis vulgaris is a medicinal plant commonly used for the treatment of numerous disorders, gastroprotective effect of its leaf extract was not investigated before.
Materials and Methods: Gastric ulcer was modelled in Sprague-Dawley rats after treatment with B. vulgaris leaf extract containing 0.07% of alkaloids, 0.48% of flavonoids and 8.05% of tanning substances, 10 or 50 mg of dry extract/kg, changes in the stomach mucosa were assessed semi-quantitatively, and the gastric wall was evaluated for prostaglandin E2 level using ELISA and assessed histologically by calculation of the lesion index.
Results: B. vulgaris leaf extract at the dose of 50 mg/kg reduced the macroscopic ulcer score and the microscopic lesion index, increased prostaglandin E2 concentration in the gastric wall significantly higher than atropine and B. vulgaris leaf extract 10 mg/kg.
Conclusion: The gastroprotective effect of the high dose of B. vulgaris leaf extract may be due to stimulation of prostaglandin E2 secretion in the stomach, and anti-oxidative and anti-inflammatory properties of polyphenolic complex of flavonoids and tannins present in the leaves of this plant.
