Title of article :
Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line
Author/Authors :
Faraji ، Parviz Department of Persian Medicine - School of Traditional Medicine, Student Research Committee - Tabriz University of Medical Sciences , Araj-Khodaei ، Mostafa Department of Persian Medicine - School of Traditional Medicine, Neurosciences Research Center, Aging Research Institute - Tabriz University of Medical Sciences , Jafari ، Amir Pharmaceutical Analysis Research Center - Tabriz University of Medical Sciences , Ghaffari ، Maryam Student Research Committee - Tabriz University of Medical Sciences , Mohammadinasab ، Reza Department of History of Medicine - School of Traditional Medicine - Tabriz University of Medical Sciences , Hamedeyazdan ، Sanaz Department of Pharmacognosy - Faculty of Pharmacy - Tabriz University of Medical Sciences , de la Guardia ، Miguel Department of Analytical Chemistry - University of Valencia , Ezzati Nazhad Dolatabadi ، Jafar Drug Applied Research Center - Tabriz University of Medical Sciences
From page :
189
To page :
194
Abstract :
Objectives: One of the well-accepted beliefs about natural products, considering the advances of recently appearing new edges and features of herbal medicine, is paying more attention to cancer treatments. However, they have not been properly studied with reasonable/reliable clinical trials in human subjects in most cases. Therefore, seeking in vitro effects of herbs like Melissa officinalis (MO) in cancer therapy to identify the involved possible mechanism in conjugation with configurative/morphological aspects of treated cells seems quite necessary. In this study, we evaluated the co-treatment effect of anti-cancer drug methotrexate (MTX) and MO on HeLa cancer cells. Methods: MTT assay was applied to assess the quantitative cytotoxicity effect of both MTX and Mo. Apoptosis assay via flow cytometry was used to determine the amount of apoptotic and necrotic cells. To further investigate the anti-cancer effects, DAPI staining and DNA ladder assays are used qualitatively to detect changes in the nuclei of cells that are a sign of apoptosis occurring and morphological modifications of DNA. Results: MTX and MO mixture showed high cytotoxicity and apoptosis rate compared to untreated cells. Furthermore, the morphological changes of MTX and MO mixture were more evident than that of single MO, MTX, and control groups. Conclusions: These data regarding cell growth reduction and apoptosis induction in HeLa cancer cells showed that MTX and MO mixture can be an appropriate platform for cancer therapy.
Keywords :
Methotrexate , Melissa officinalis , HeLa cancer cells , MTT assay , Flow cytometry
Journal title :
Crescent Journal of Medical and Biological Sciences
Journal title :
Crescent Journal of Medical and Biological Sciences
Record number :
2779486
Link To Document :
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