Title of article :
Sequential changes in expression of long non-coding RNAs THRIL and MALAT1 after ischemic stroke
Author/Authors :
Bayat ، Mahnaz Clinical Neurology Research Center - Shiraz University of Medical Sciences , Hooshmandi ، Etrat Clinical Neurology Research Center - Shiraz University of Medical Sciences , Karimi ، Najmeh Iran Department of Neurology - Clinical Neurology Research Center - Shiraz University of Medical Sciences , Rahimi ، Moosa Laboratory of Basic Sciences - Mohammad Rasul Allah Research Tower - Shiraz University of Medical Sciences , Tabrizi ، Reza Noncommunicable Diseases Research Center - Fasa University of Medical Sciences , Asadabadi ، Tahereh Clinical Neurology Research Center - Shiraz University of Medical Sciences , Salehi ، Mohammad Saied Clinical Neurology Research Center - Shiraz University of Medical Sciences , Zafarmand ، Shaghayegh Clinical Neurology Research Center - Shiraz University of Medical Sciences , Owjfard ، Maryam Clinical Neurology Research Center - Shiraz University of Medical Sciences , Garcia Esperon ، Carlos Department of Neurology - John Hunter Hospital , Spratt ، Neil Department of Neurology - John Hunter Hospital , Levi ، Christopher Hunter Medical Research Institute - University of Newcastle , Borhani-Haghighi ، Afshin Clinical Neurology Research Center - Shiraz University of Medical Sciences
From page :
74
To page :
82
Abstract :
Background: Inflammation is the major contributor to the pathophysiology of ischemic stroke (IS). Long non-coding ribonucleic acids (lncRNAs) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and tumor necrosis factor and heterogeneous nuclear ribonucleoprotein L-related immunoregulatory (THRIL) have been demonstrated to be up-regulated in inflammation and atherosclerosis. Therefore, we aimed to study the expression profile of these lncRNAs after IS.Methods: This observational case-control study was conducted in Namazi Hospital, Shiraz, Iran. The real-time polymerase chain reaction (RT-PCR) measured the sequential changes in circulating levels of MALAT1 and THRIL on days 1, 3, and 5 after IS. The receiver operating characteristic (ROC) curve analysis was used to estimate the diagnostic and prognostic potential of lncRNAs with the area under the curve (AUC).Results: In patients with IS, the relative MALAT1 and THRIL expressions were significantly higher than the controls (P 0.001 and P 0.01, respectively), on days 1, 3, and 5 after stroke. We showed a significantly increase in lncRNAs expression on day five compared to days 1 and 3 after stroke. Moreover, a positive correlation was detected between MALAT1 expression and time within the first 24 hours after stroke (r = 0.27, P = 0.03). Logistic regression analysis showed a significant positive association between MALAT1 and THRIL and the risk of stroke evolution. We found a potential diagnostic marker for MALAT1 with an AUC of 0.78.Conclusion: We demonstrated the significant sequential upregulation in MALAT1 and THRIL expression on days 1, 3, and 5 after IS with a significant positive association with the risk of stroke. MALAT1 also significantly correlated with time within the first 24 hours after stroke.
Keywords :
Long Noncoding RNA , MALAT1 Long Noncoding RNA , THRIL Long Noncoding RNA , Ischemic Stroke
Journal title :
current journal of neurology
Journal title :
current journal of neurology
Record number :
2779595
Link To Document :
بازگشت