Nanosized TiO2 Induced Ovarian Alterations in NMRI Mice Treated with Isoniazid

Titanium dioxide (TiO2) is a widely used material in various products, including cosmetics, food additives, and medical devices. However, its small size (nanoscale) has raised concerns about its potential impacts on human health and the environment. One area of concern is the effect of nanosized TiO2 on the female reproductive system, particularly the ovaries. The purpose of this study is to look at how isoniazid-treated NMRI mice’s ovarian tissue alters in response to nanosized titanium dioxide (nano-TiO2) treatment. The 50 adult female mice used in this research were split into five groups of 10 at random. The experimental groups included the control group (no medication), sham group (0.5 mg/kg of normal saline), first experimental group (45 mg/kg isoniazid), second experimental group (45 mg/kg isoniazid and 0.5 mg/kg nano-TiO2), and the third experimental group (45 mg/kg isoniazid and 0.45 mg/kg nano-TiO2). All injections were given for 20 days. After that, the ovarian tissue from each animal was isolated and put in an 10% formalin solution before tissue analyses were carried out using hematoxylin and eosin (H E) stain. In comparison to the control group, there was a reduction in the quantity of corpus luteum and secondary follicles in all experimental groups. In comparison to the first experimental group, there is a significant increase in the number of unilaminar primary follicles in the rest of the experimental groups. The damaging effects of isoniazid on ovarian tissue can be lessened by using nanoparticles in small amounts.