Title of article :
Comparison of Mir122 expression in children with biliary atresia and healthy group
Author/Authors :
Motazedian ، Nasrin Transplant Research Center - Shiraz University of Medical Sciences , Azarpira ، Negar Transplant Research Center - Shiraz University of Medical Sciences , Falamarzi ، Kimia Student Research Committee - Shiraz University of Medical Sciences , Dehghani ، Mohsen Transplant Research Center - Shiraz University of Medical Sciences , Ataollahi ، Maryam Transplant Research Center - Shiraz University of Medical Sciences , Esfandiari ، Elaheh Transplant Research Center - Shiraz University of Medical Sciences , Dara ، Mahintaj Stem Cells Technology Research Center - Shiraz University of Medical Sciences , Toobafard ، Razieh Transplant Research Center - Shiraz University of Medical Sciences , Sayadi ، Mehrab Cardiovascular Research Centre - Shiraz University of Medical Sciences , Shekarforoush ، Ali Transplant Research Center - Shiraz University of Medical Sciences , Owji ، Hossein Department of Otolaryngology - Student Research Committee, Otolaryngology Research Center - Shiraz University of Medical Sciences , Malekhosseini ، Ali Abu-Ali Sina Organ Transplant Center - Shiraz University of Medical Sciences
Abstract :
Biliary atresia (BA) is the primary cause of neonatal jaundice with various pathological mechanisms. Many BA patients may experience progressive liver dysfunction and eventually need a liver transplant. Therefore, identifying potential non-invasive biomarkers for BA is crucial. miR-122, the most abundant microRNA in the liver, plays significant roles in different liver diseases. This study aimed to assess miR-122 levels in BA patients. Eighteen patients with biliary atresia were selected at random from the Shiraz Pediatric Liver Cirrhosis Cohort Study (SPLCCS), along with 18 healthy controls. Blood samples were collected, and biochemical parameters (such as liver function tests) were measured. Quantitative reverse-transcription PCR (RT-PCR) was conducted on serum samples from both the case and control groups to analyze miR-122 levels. The study results indicated that serum miR-122 expression in BA patients was elevated compared to the control group, although it did not reach statistical significance. Additionally, no correlation was found between miR-122 expression and serum levels of liver enzymes or other laboratory findings in BA cases. miR-122 could be a potential target for diagnosing BA; however, further research with a larger population is necessary to determine if miR-122 could serve as a useful biomarker for diagnosing BA.
Keywords :
Micro RNA , miR , 122 , Biliary atresia , Liver
Journal title :
Molecular Biology Research Communications
Journal title :
Molecular Biology Research Communications
