Title of article :
Analyzing Antibiotic Resistance in Clinical Mycobacterium tuberculosis Isolates using Microplate Alamar Blue Assay
Author/Authors :
Ahmadi ، Kamal Department of Mycobacteriology and Pulmonary Research - Microbiology Research Center (MRC), Student Research Committee - Pasteur Institute of Iran , Madadi-Goli ، Nahid Department of Mycobacteriology and Pulmonary Research - Microbiology Research Center (MRC), Student Research Committee - Pasteur Institute of Iran , Masoumi ، Morteza Department of Mycobacteriology and Pulmonary Research - Microbiology Research Center (MRC) - Pasteur Institute of Iran , Nasehi ، Mahshid Department of Epidemiology and Biostatistics - School of Public Health - Iran University of Medical Sciences , Siadat ، Davar Department of Mycobacteriology and Pulmonary Research - Microbiology Research Center (MRC) - Pasteur Institute of Iran , Vaziri ، Farzam Department of Mycobacteriology and Pulmonary Research - Microbiology Research Center (MRC) - Pasteur Institute of Iran , Fateh ، Abolfazl Department of Mycobacteriology and Pulmonary Research - Microbiology Research Center (MRC) - Pasteur Institute of Iran
From page :
174
To page :
178
Abstract :
Introduction: Tuberculosis, caused by Mycobacterium tuberculosis, is one of the most common infectious diseases worldwide. Epidemiological studies of M. tuberculosis drug resistance are critical for improving patient treatment approaches and controlling the spread of tuberculosis. The present study aimed to determine antibiotic resistance among M. tuberculosis clinical isolates using the Microplate Alamar Blue Assay (MABA). Methods: In this descriptive cross-sectional study, 25 M. tuberculosis isolates from clinical samples were identified and confirmed using standard microbiological and biochemical tests. Then, the MIC for the antibiotics Bedaquiline, isoniazid, rifampin, ethambutol, ofloxacin, moxifloxacin, capreomycin, and streptomycin was determined using the MABA method. The results were analyzed using SPSS version 16 software. Results: Among the 25 investigated isolates, the frequencies for MDR, Pre-XDR, and XDR isolates were 20%, 8%, and 32%, respectively. The highest rate of drug resistance was to isoniazid (80%), rifampicin, and ethambutol (76%), and the highest rate of sensitivity was to moxifloxacin (68%). The frequency of isoniazid mono-resistance and rifampicin mono-resistance was 5 cases (50%) and 4 cases (40%), respectively. Conclusion: Our study revealed an alarming rate of MDR and XDR M. tuberculosis strains, indicating that current first-line treatments may be ineffective for a significant number of patients. The bedaquiline resistance among the isolates with no history of previous exposure to this drug suggests unexplored resistance mechanisms. Molecular techniques to accurately identify these mechanisms may contribute to developing more effective treatment strategies to combat drug-resistant tuberculosis.
Keywords :
Mycobacterium tuberculosis , Bedaquiline , Microplate Alamar Blue Assay , Multi , drug resistance , Extensive drug , resistant tuberculosis
Journal title :
Journal of Medical Microbiology and Infectious Diseases
Journal title :
Journal of Medical Microbiology and Infectious Diseases
Record number :
2781119
Link To Document :
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