Title of article :
A new theranostic complex based on bismuth-iron oxide nanoparticle for myocardial ischemia: in vitro study
Author/Authors :
Kordani ، Mohammad Reza Department of Medical Nanotechnology - Faculty of Advanced Sciences and Technology - Islamic Azad University, Tehran Medical Sciences branch , Hataminia ، Fatemeh Department of Medical Nanotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences , Karimi ، Fateme Department of Medical Nanotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences , Motakef Kazemi ، Negar Department of Medical Nanotechnology - Faculty of Advanced Sciences and Technology - Islamic Azad University, Tehran Medical Sciences branch , Ghanbari ، Hossien Department of Medical Nanotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences
Abstract :
Objective(s): Magnetic nanoparticles of bismuth oxide and iron oxide (BOIO NPs) are extensively used for more accurate medical diagnosis in MRI and CT imaging due to their improved X-ray absorbance and magnetic properties. The nanoparticles (NPs) utilized in the diagnosis of damaged tissue can also be considered an improvement factor in treatment. In this study, an anti-inflammatory drug, indomethacin, was loaded on BOIO NPs.Methods: The BOIO NPs were synthesized using the hydrothermal precipitation method. To ensure stable dispersion, the NPs were coated with silica-polyethylene glycol (PEG). Indomethacin was also used to alleviate the initial inflammation. The drug was loaded on the PEG shell with a solvent.Results: Cytotoxicity and anti-inflammatory tests were carried out on H9C2 cells and H2O2 treated cells. MRI and CT signal amplification was evaluated in the phantom. In MRI and CT, the signal surge of these NPs investigated at four concentrations (0, 0.85, 1.7, 3.4 mg/ml) by significant differences (P 0.05). The best drug loading efficiency (DLE) and drug loading content (DLC) were measured as 58.5 ±8 % and 10.5 ±1.3 % respectively. Invitro drug release evaluated after 72 h was about 44.08±0.01%. Conclusions: According to the results of MRI and CT techniques and signal amplification relative to water for PEGsi-BOIO NPs, it is concluded that these NPs could be employed to track any inflammated tissue. The cytotoxicity results demonstrated the improvement of the induced reactive oxygen species (ROS) in H9C2 tissue cells and its capability through the delivery of indomethacin.
Keywords :
Pegylated bismuth oxide–iron oxide NPs , MRI technique , CT , Indomethacin , Myocardial infarction , Drug loading
Journal title :
Nanomedicine Research Journal
Journal title :
Nanomedicine Research Journal