Title of article
Pressure-dependent Activity of Dihydrofolate Reductase from a Deep-sea Bacterium Shewanella violacea Strain DSS12
Author/Authors
Gekko، Kunihiko نويسنده , , Ohmae، Eiji نويسنده , , Kubota، Kazumasa نويسنده , , Nakasone، Kaoru نويسنده , , Kato، Chiaki نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
-797
From page
798
To page
0
Abstract
A new dihydrofolate reductase (svDHFR) was purified from a deep-sea bacterium, Shewanella violacea strain DSS12, isolated from the Ryukyu Trench (5110 m). In contrast with E. coli DHFR, the enzyme activity of svDHFR increased with increasing hydrostatic pressure up to 100 MPa, suggesting that the enzyme kinetics and structural fluctuation of svDHFR are adapted to a high-pressure environment.
Keywords
prediction , Genetic-fuzzy system , Application-production research , Surface finish , grinding , Power requirement
Journal title
CHEMISTRY LETTERS
Serial Year
2004
Journal title
CHEMISTRY LETTERS
Record number
28523
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