Improved Diagnosis of Sickle Cell Mutation by a Robust Amplification Refractory Polymerase Chain Reaction

Background: The aim of this study was to investigate whether BGD activity is of additional value in the assessment of pulmonary inflammation caused by coal dust exposure. Design and methods: Ex-coalminers were included in this study. Forty-eight healthy male subjects, without a relevant medical history, were used as controls. Results: In ex-coalminers serum BGD activity was higher compared to the control group. Moreover, ex-coalminers with a normal chest radiograph and normal serum LDH demonstrated elevated serum BGD compared to the control group. However, no relation was found in the total group of ex-coalminers between serum BGD activity and pulmonary function parameters. Conclusions: Our study adds in vivo human evidence to the already existing animal data that BGD is a potential biomarker useful in monitoring pulmonary inflammation caused by coal dust exposure. Copyright © 1999 The Canadian Society of Clinical Chemists