Title of article
FLUOROMETRIC DETERMINATION OF PEROXIDES IN AN HPLC SYSTEM
Author/Authors
Hu، Yi-Min نويسنده , , Qi، Bin نويسنده , , Zhu، Y. Z. نويسنده , , Zhang، Yuanhang نويسنده , , Tang، Xiaoyan نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2001
Pages
-1246
From page
1247
To page
0
Abstract
Hemin was used to substitute for horseradish peroxidase (HRP) as catalyst with phydroxyphenyl acetic acid (PHPAA) as substrate for post-column detection of H2O2 and methyl hydroperoxide (CH3OOH, MHP) in an HPLC system. In a hemin-catalyzed system, post-column reaction and fluorescence measurement can both be conducted optimally at a pH of ~10.5. Hydroxymethyl hydroperoxide (HOCH2OOH, HMHP) and bis(hydroxymethyl) peroxide (HOCH2OOCH2OH, BMHP) will rapidly be converted to H2O2 in alkaline solution and can also be detected. The HPLC system is optimized for the analysis of H2O2 and MHP. Reproducibilities are better than 5% for H2O2 and MHP. The detection limits in aqueous solution are 9 nM for H2O2 and 200 nM for MHP.
Keywords
Non-linear chromatography , separation , System identification
Journal title
Analytical Letters
Serial Year
2001
Journal title
Analytical Letters
Record number
36973
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