On the kinetics and effectiveness of immobilized whole-cell batch cultures

A model for growth-associated fermentations is presented for batch cultures of immobilized whole cells. The model encompasses exponential and steady-state growth phases and the effectiveness of the biocatalyst. Based on the model equations, experimental methods have been developed for the determination of the kinetic parameters: maximum specific growth rate (μmax), Monod constant (Km) and effectiveness factor (η). These methods are illustrated by ethanol fermentations using Saccharomyces cerevisiae cells entrapped in alginate beads. The kinetic parameters obtained show consistency with those determined for free-cell systems.