Title of article :
Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1
Author/Authors :
R.S. Singh، نويسنده , , Balwinder Singh Sooch، نويسنده , , Munish Puri، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Abstract :
A newly isolated strain of Kluyveromyces marxianus YS-1 was used for the production of extra cellular inulinase in a medium containing inulin, meat extract, CaCl2 and sodium dodecyl sulphate (SDS). Fermentation medium pH 6.5, cultivation temperature 30 °C and 5% (v/v) inoculum of 12 h-old culture were optimal for enzyme production (30.8 IU/ml) with a fermentation time of 72 h at shake flask level. Raw inulin (2%, w/v) extracted from dahlia tubers by processing at 15 kg/cm2 for 10 min was optimum for bioreactor studies. Maximum enzyme production (55.4 IU/ml) was obtained at an agitation rate of 200 rpm and aeration of 0.75 vvm in a stirred tank reactor with a fermentation time of 60 h.
Keywords :
Kluyveromyces marxianus , Medium optimization , inulinase , inulin
Journal title :
Bioresource Technology
Journal title :
Bioresource Technology