Title of article :
Partial Molecular Characterization of a Czech Isolate of Grapevine leafroll-associated virus 3
Author/Authors :
P. Kom?nek، نويسنده , , M. Bryxiov? and M. Glasa، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Abstract :
Grapevine leafroll-associated virus 3 (GLRaV-3) is an
important pathogen of grapevine in the Czech Republic.
A Czech isolate was studied at the molecular level
in order to extend data on molecular variability of the
virus from the central European region. Three conserved
regions (genes encoding the RNA-dependent
RNA polymerase, the heat shock protein 70 and the
55 kDa protein) were amplified using reverse transcriptase-
polymerase chain reaction, sequenced and compared
with related available sequences. On the
sequenced regions, the Czech isolate revealed more
than 99% nucleotide and amino acid identity with the
published full-length sequence of a North American
GLRaV-3 isolate and a similarly high identity with
available partial GLRaV-3 sequences of isolates from
different parts of the world. The phylogenetic position
of the new and already characterized GLRaV-3 isolates
within the Ampelovirus genus is discussed.
Keywords :
HSP 70 gene , 55 kDa protein gene , Czech Republic , Grapevine leafroll-associated virus 3 , polymerase gene , ampelovirus
Journal title :
Journal of Phytopathology
Journal title :
Journal of Phytopathology