Author/Authors :
Kazuhiro Nakamura، نويسنده , , Ryunosuke Ohkawa، نويسنده , , Shigeo Okubo، نويسنده , , Minoru Tozuka، نويسنده , , Mitsumasa Okada، نويسنده , , Shinya Aoki، نويسنده , , Junken Aoki، نويسنده , , Hiroyuki Arai، نويسنده , , Hitoshi Ikeda، نويسنده , , Yutaka Yatomi، نويسنده ,
Abstract :
Objectives:
Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing.
Design and methods:
LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate.
Results and conclusions:
It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.
Keywords :
lysophosphatidylcholine , Plasma , Lysophospholipase D , Autotaxin , Lysophosphatidic acid