Title of article :
Immunophenotypic Aberrations, DNA Content, and Cell Cycle Analysis of Plasma Cells in Patients with Myeloma and Monoclonal Gammopathies
Author/Authors :
Margarida Lima، نويسنده , , Maria dos Anjos Teixeira، نويسنده , , Sonia Fonseca، نويسنده , , Cristina Gonçalves، نويسنده , , Marisol Guerra، نويسنده , , Maria Lu?s Queiros، نويسنده , , Ana Helena Santos، نويسنده , , Antonio Coutinho، نويسنده , , Luciana Pinho، نويسنده , , Luc?lia Marques، نويسنده , , Manuel Cunha، نويسنده , , Pinto Ribeiro، نويسنده , , Luciana Xavier، نويسنده , , Herm?nia Vieira، نويسنده , , Pureza Pinto، نويسنده , , Benvindo Justiça، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2000
Abstract :
We describe the immunophenotypic and gross DNA defects in 55 patients with myeloma and 50 patients with monoclonal gammopathy and review the literature on this subject (MedLine, 1994–2000). Our data confirmed previous reports indicating that in myeloma nearly all marrow plasma cells are abnormal (98.7 ± 8.1%). In monoclonal gammopathy the fraction of abnormal plasma cells was 35.0 ± 32.8%. In both myeloma and monoclonal gammopathy, the most frequent aberrant phenotypic features consisted of absence of expression of CD19, strong expression of CD56, and decreased intensity of expression of CD38; aberrant expression of CD10, CD20, CD22, or CD28 was observed in less than one-third of myeloma cases. The vast majority of cases had two or more phenotypic aberrations. In the DNA studies, 7% of myeloma cases were biclonal and 93% of cases were monoclonal. In those studies with only one plasma cell mitotic cycle, 37% had normal DNA content and 63% were aneuploid (hyperploid, 61%; hypoploid, 2%). The mean percentages of plasma cells in S- and G2M phases were 4.9 ± 8.5 and 4.4 ± 6.9%, respectively. Thirty-eight percent of cases had more than 3% of plasma cells in S phase. In monoclonal gammopathy, the DNA index of abnormal plasma cells ranged from 0.89 to 1.30 and the percentage of diploid (31%) and aneuploid (69%) cases was not different from the results found in myeloma. The differences in percentage of abnormal plasma cells in S- (7.4 ± 8.6%) and G2M-phases (2.4 ± 1.7%) in patients with monoclonal gammopathy were not statistically significant.
Keywords :
plasma cells , DNA , immunophenotype , myeloma , monoclonal gammopathy
Journal title :
Blood Cells, Molecules and Diseases
Journal title :
Blood Cells, Molecules and Diseases