Title of article :
A flow-through enzyme immunoassay for the screening of fumonisins in maize
Author/Authors :
Paepens، C. نويسنده , , Saeger، S. De نويسنده , , Sibanda، L. نويسنده , , Barna-Vetro، I. نويسنده , , Leglise، I. نويسنده , , Hove، F. Van نويسنده , , Peteghem، C. Van نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
-228
From page :
229
To page :
0
Abstract :
The format of an existing flow-through enzyme immunoassay has been optimised for the detection of fumonisin B1 (FB1) and B2 (FB2) in maize. The visual detection limit i.e. the smallest mycotoxin concentration resulting in no colour development, is 1000 (mu)g/kg. Assay validation was performed using samples spiked with respectively only FB1 and a FB1 + FB2 mixture (ratio 1:1). Results showed that within-day and between-day coefficients of variation ranged from respectively 0.4– 10.2% and 1.1–9.0%. Naturally contaminated samples were screened with the developed flow-through method and results were compared with fumonisin contamination values obtained by a validated HPLC method. The assay was demonstrated to be accurate and reliable giving no false compliant and only a low percentage of false non-compliant results. The described method offers a simple, rapid and cost-effective screening tool, thus contributing to a better consumers’ health protection.
Keywords :
Rapid screening method , Fumonisins , HPLC
Journal title :
Analytica Chimica Acta
Serial Year :
2004
Journal title :
Analytica Chimica Acta
Record number :
50513
Link To Document :
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