Single Pass Sequencing of a Unidirectional Human Fetal Heart cDNA Library to Discover Novel Genes of the Cardiovascular System

A human fetal heart cDNA library was constructed in the lambda gt22A expression vector. Polymerase chain reaction (PCR) was used to amplify the cDNA inserts. PCR products were purified and used in cycle sequencing reaction in the presence of a fluorescein-conjugated primer and electrophoresed on a Pharmacia A.L.F. Sequencer, Partial cDNA sequences, or expressed sequences tags (ESTs) were searched against the Genbank and EMBI, databases to identify novel genes expresses in the human cardiovascular system