Author/Authors :
Donguk Kim، نويسنده , , Tae-young Roh، نويسنده , , Jieun Lee، نويسنده , , Joo-yoon Noh، نويسنده , , Young-Joo Jang، نويسنده , , Kwang-Lae Hoe، نويسنده , , Hyang-Sook Yoo، نويسنده , , Myung-Un Choi، نويسنده ,
Abstract :
We cloned and expressed a full-length cDNA encoding a phospholipase D of type α (PLDα) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.
Keywords :
phospholipase d , cDNA sequence , expression , (Brassica oleracea var. capitata)
