Inclusion Bodies Contraction with Implications in Biotechnology

Irreversible contraction of inclusion bodies was found to have a huge impact on sedimentation velocity, solubility and extractability of target protein from non-classical inclusion bodies. The downstream process during protein isolation from non-classical IBs is also affected. Based on extractability studies accompanied by electron microscopy, a hypothetical model of IBs formation mechanism is presented, which is supported by usually observed single IB per E. coli cell.