Title of article
Characterization of cytosolic glutathione S-transferases in California Halibut (Paralichthys californicus)
Author/Authors
Rachel T. Donham، نويسنده , , Andrea D. Luna، نويسنده , , Sandra Chang، نويسنده , , Dexter Morin، نويسنده , , William T. Jewell، نويسنده , , Ronald S. Tjeerdema، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
6
From page
133
To page
138
Abstract
Two cytosolic glutathione S-transferase (GST) classes were isolated and characterized from California halibut (Paralichthys californicus) liver. Nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to elucidate peptide sequences and the proteins were identified as θ and α by searching against the NCBI non-redundant database (nrDB). Catalytic activity of the cytosolic GSTs towards 1-chloro-2,4-dinitrobenzene (CDNB) was determined to be 0.23±0.003 U/mg cytosolic protein.
Keywords
glutathione S-transferase , California halibut , Paralichthys californicus
Journal title
Ecotoxicology and Environmental Safety
Serial Year
2007
Journal title
Ecotoxicology and Environmental Safety
Record number
711167
Link To Document