Operating conditions for the determination of the biochemical acidogenic potential of wastewater

The aim of this work was to study the test conditions for the determination of the biochemical acidogenic potential (BAP) of wastewater, which should be useful to predict the performance of enhanced biological phosphorus removal (EBPR). Proposed operating conditions for a simple and reproducible BAP test in 250 ml serum bottles (equipped with black butyl stoppers and magnetic bars) are: use of either frozen or fresh water, no inoculum addition, fermentation carried out in the dark during 15 days, addition of 1 mM bromo-ethane sulfonate (BES) and 2 mM barium chloride (BaCl2), stirring speed strong enough to maintain vortex conditions, no pH control and controlled temperature of 20°C.