Identification of the parental lines and hybrids of rice (Oryza sativa L.) using RAPD markers

Application of RAPD markers for the identification of three rice (Oryza sativa L.) hybrids and their parental lines i.e. CMS female parent (A line), maintainer (B line) and pollen parent (R line), was tested using 17 random oligonucleotides (Operon Technologies, USA). It was possible to distinguish each of these genotypes, following a combination of selected primers. The results are discussed in view of its application for the purpose of Plant Variety Protection and for testing the genetic purity of A line and hybrid seed lots.