Author/Authors :
Elena N. Sigida، نويسنده , , Yuliya P. Fedonenko، نويسنده , , Alexander S. Shashkov، نويسنده , , Evelina L. Zdorovenko، نويسنده , , Svetlana A. Konnova، نويسنده , , Vladimir V. Ignatov، نويسنده , , Yuriy A. Knirel، نويسنده ,
Abstract :
Lipopolysaccharide was obtained by phenol–water extraction from dried bacterial cells of Azospirillum brasilense type strain Sp7. Mild acid hydrolysis of the lipopolysaccharide followed by GPC on Sephadex G-50 resulted in a polysaccharide mixture, which was studied by composition and methylation analyses, Smith degradation and 1H and 13C NMR spectroscopy. The following polysaccharide structures were established, where italics indicate a non-stoichiometric (∼40%) 2-O-methylation of l-rhamnose.