14040

Determination of Juglone (5-hydroxy 1,4-naphthoquinone) in Pterocarya fraxinifolia by RP-HPLC

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A methodfor determination ofjugIone in leaves and hulls ofPterocaryafraxinifolia was developed, based on RP-HPLC. This compound was extracted from leaves and hulls by chloroform refluxing about 2 hours. The chloroform was evaporated under low pressure at 50 °C to produce a dry residue. The remained materials were dissolved in acetonitrile to be used for determination of this compound. Appropriate conditions for RP-HPLC were determined using standard of juglone. These conditions include, acetonitrile percentage in mobile phase, pH of mobile phase (phosphate buffer) and temperature of column, which were 50%, 4 and 30°C, respectively. Juglone concentration was determined using standard addition method. The concentrations ofjuglone were 2.15, 2. 74,1. 77,J.J2and 0.34 gin 100 g ofdry leaves in May, June, July, August and September 2001, respectively. The content ofjuglone in hulls was 0.44 g in JOO g ofdry hulls in May.

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