عنوان به زبان ديگر :
Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil
پديد آورندگان :
Falahatpishe Hamidreza نويسنده , Jalali Mahmoud نويسنده , Badami Naser نويسنده , Mardani Nadia نويسنده , Khosravi-Darani Kianoush نويسنده
چكيده لاتين :
This research has focused on isolation and characterization of a strain of Bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at pHs ranging from 8 to 11 and temperatures of 20 to 50°C. Also the impact of different carbon and nitrogen sources were investigated. The yield and fold of enzyme purification was 24% and 50 times, respectively. Molecular weight of purified enzyme was measured by SDS-PAGE as 24.7 kDa. The alkaline protease produced by Bacillus sp. 2 - 5 showed the most caseinolytic activity (without any gelatinolytic activity) atpH>10.
