عنوان به زبان ديگر :
Evaluation of a Native Preparation of HCV Core Protein (2-122) for Potential Applications in Immunization, Diagnosis and mAb Production
پديد آورندگان :
Aghasadeghi MR نويسنده , Sadat S M نويسنده , Budkowska A نويسنده , Khabiri A R نويسنده , Amini S نويسنده , Bahramali G نويسنده , Naddaf S R نويسنده , Roohvand F نويسنده
چكيده لاتين :
Infection with hepatitis C virus (HCV) is a worldwide problem. Among HCV proteins, core antigen (Ag), besides its importance
for diagnostic application is a prime candidate for component of a vaccine. Herein, we report results of studies on
production of the hydrophilic domain of core Ag (2-122) in native conformation by an arabinose induction system in E.coli
and the primary characterization of this recombinant protein for applications in diagnosis, immunization and mAb production.
Recombinant core (r-Core) was able to detect anti-core antibodies in HCV positive serum samples in a dilution rate of
1/3200. It was also capable to elicit a potent anti-HCV humoral immune response in BALB/c mice. Finally, we established
two stable clones of hybridoma which shown to produce specific and sensitive mAbs against the core protein. HCV core
was able to elicit a broad range of antibody specificities depending on the immunogen conformation. Therefore, it may be
possible to get new mAbs with higher affinities towards native conformation of core Ag.
