پديد آورندگان :
Zarghi Afshin نويسنده , Shafaati Alireza نويسنده , KHODDAM ARASH نويسنده , FOROUTAN SEYYED MOHSEN نويسنده
چكيده لاتين :
A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for
the determination of risperidone in human plasma was developed. An HPLC system based on
a Nucleosil Cs column (l50x4 mm) and a UV detector (11,= 280 nm) were used. A mixture of
sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate
of 1.5 ml minי was used as mobile phase. The proteins were precipitated with an acetonitrile
solution containing diltiazem as internal standard and the average recovery was 93.9±3.4%.
The detection limit for risperidone in plasma was 0.5 ngml". The calibration curve was linear
over the concentration range 2-50 ngmlי. The inter-day and intra-day assay coefficients of
variation were found to be less than 5%. The present validated method was successfully used
for pharmacokinetic studies of risperidone in human subjects.
