چكيده لاتين :
Bacterial protein toxins have been exploited as therapeutic agents and as vaccines. An issue
of deserving interest is development of new generations of vaccines and immune adjuvants.
In this study an active assembled recombinant Shiga toxin of Escherichia coli (rStxl) and its
derivatives, recombinant A and B subunits (Stxl-A and Stxl-B), were used to immunize
mice. The elicited antibody responses were compared with and without using adjuvant.
Protection against intraperitoneal lethal dose of rStx challenge was observed by
immunization with sublethal dose of rStxl, rStx-A and rStx-B subunits.
The immunological studies on toxin subunits can be used for immunization against
systemic shiga toxin mediated disease and also subunits as a vector for antigen presentation in
immunotherapeutic approaches. In our experiment, while stimulation of the immune system by
A and B subunits were different, both subunits produced neutralizing antibodies. Regarding B
subunit the amount of specific IgG I/IgG2a antibody ratio was higher than A subunit.
In addition B subunit stimulated proliferation of immune cells with IFNy production the
same as rStxl, suggesting that B subunit can be used as an immunomodulator to stimulate
the immune response in conjunction with other recombinant proteins
