چكيده لاتين :
Hepatitis C is a global health problem. The exact mechanisms by which hepatitis C virus (HCV) can evade the host immune system have become controversial. Whether HCV polyproteins modulate IFN signalling pathways or HCV proteins are responsible for such a property is the subject of interest. Therefore, an efficient baculovirus delivery system was developed to introduce the whole genome of HCV IB minus 3יuntranslated region (UTR) (HCV1 BA3יUTR) into hepatoma cells. Methods: The whole genome of HCV genotype lb was developed into hepatoma cells. Also, two replicon constructs were used in this research: a recombinant baculovirus containing the culture adapted sub-genomic replicon (FK.5.1) derived from HCV genotype lb, and a mutant form containing an inactivating mutation within the non-structural protein 5B (NS5B). Results: As expected, the baculovirus carrying the FK.5.1 replicon induced the production of IFN-0 as judged by the use of an IFN-p promoter luciferase reporter construct, whereas the GND baculovirus (a control polymerase knock-out replicon) and the full-length 3יUTR deletant failed to induce luciferase expression. The activation of both IFN regulatory factor 3 (IRF3) and nuclear factor KB (NFKB), two transcription factors induced by dsRNA signalling were examined. Both the wild type and GND-mutant replicon blocked the dsRNA-induced activation of IRF3 and NFKB. Conclusion: Inhibition of the transcriptional response to IRF3 and NFKB seems to be one of the multiple mechanisms which HCV employs to escape the host immune defence. In contrast, the full length 3יUTR deletant had no significant effect on either transcription factor. These results may be attributed to the function of HCV subgenomic replicons when compared with full length 3יUTR deletant
