مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

This study was performed to determine whether melatonin at physiological concentrations (0.01-10nM) could affect the proliferation and osteogenic differentiation of Rat ADSCs in vitro. Materials and Methods: ADSCs were isolated from the fat of adult rats. After cell expansion in culture media and through three passages , osteogenesis was induced on a monolayer culture with osteogenic medium with or without melatonin at physiological concentrations (0.01-10nM). After 4 weeks cultures were examined for mineralization by Alizarin Red S and von Kossa staining and for alkaline phosphatase (ALP) activity by ALP kit. Cell viability and apoptosis were also assayed by 3-(4, 5-dimethylth iazol-2-yl)-5-(3-carboxymethoxyphenyl)2-( 4-ulfophenyl)-2H-tetrazolium assay and flowcytometry, respectively. All assays were performed in triplicate. Results : The results indicated that at physiological concentrations, melatonin suppressed proliferation and differentiation of ADSCs . These data indicate that ADSCs exposed to melatonin, had a lower ALP activity in contrast to the cells exposed to the osteogenic medium alone . Similarly, the mineral deposition (calcium level) also decreased. The flow cytometry proved that the cell growth decreased and the apoptotic cells increased. Conclusion: These results suggest that physiological concentration of melatonin has a negative effect on ADSCs osteogenesis.